Everything That You Need To Know About Micro-Centrifuge Equipment

What is Micro-centrifuge?Microcentrifuges

They are same as the Centrifuge, the only thing is that they carry lesser amount of fluid to test-maximum 2 ml or lesser than that. The liquid samples inserted in the tube spin at a very high speed.

So, what does a micro-centrifuge do?

The main reason they are used is to centrifuge nucleic acid and protein. When we say nucleic acid and protein, it means DNA and RNA. These tests are run in molecule laboratory. Amongst other clinical laboratory it is used excessively in molecule biology section. The samples tested here are very small in quantity, but the big research depends on these quantity only. These tests are performed using Eppendorf tubes. These tubes are specifically made for molecular labs only. These tubes are used in PCR laboratories as well.

Understanding Functions

Tubes

The tubes inserted in the equipment are small in size. They are built up of plastic, and they are supposed to discard after one use. They are cylindrical in shape. The tubes have different sizes as per the fluid quantity. They come in 0.5 ml, 1.5 ml, 0.2 ml and 2 ml size. The most common tube size is 1.5ml. Disinfection of the tube is possible, but due to small size they are difficult to clean, but inexpensive. So it is better to purchase new one.

Equipment

The maximum speed of this equipment is 13000 to 15000 RPM (round per minute). The equipment has a button that allows you to set the speed. Alike, centrifuge equipment the balancing of tubes is important-technically it is called sample loading balancing. By balancing we also mean that the quantity we are inserting in one tube should be similar in remaining tubes. Which means if one tube has 1.5 ml fluid, other tubes must consist around same quantity. Sometimes it is seen that the laboratory person has only one sample to test. In that case he can insert water or oil of the same density of the sample in other tubes and run the test.

Rotor

There are two types of rotor;

  • Fixed Angle Rotor
  • Swinging Bucket Rotor

The rotor lids come in two types, one is plastic which is helpful in avoiding corrosions and are transparent. Second is built up of steel which is easy to use and much safer. Both have their own advantages, however, metal rotor lids are recommended.

Safety Measures

Sometimes during the test, the equipment starts vibrating. To avoid such cases try to keep surface in level.

Introducing NeuationNeuation offers a microcentrifuges

Neuation offers a wide range of next generation microcentrifuges for various applications in research labs for processing low volume samples.

  • Highly efficient
  • Smart
  • Best work for biotech/PCR work, DNA/RNA extractions
  • Speeds up to 15000 rpm.

The available models are, iFuge M08, iFuge BL08 VT, iFuge M12P, iFuge M18P.

To know more about it, visit the page, http://www.neuation.com/microcentrifuges_new.html

A Guide to Help by the Appropriate Microcentrifuge

Medical-CentrifugeAll most every medical laboratory will have a Microcentrifuge device; the main reason being that there are many uses of Microcentrifuge device. It is very common for a chemical and biological laboratory to have one too. This is one device that is used to a great extent in such labs and for many other applications as well.

Since a Microcentrifuge is so commonly used, there are several types of them available. This article goes on to explain the subtle differences and special choices that you can make when need to buy centrifuge.

The Tube:

A Microcentrifuge can be used with a wide range of tubes. The tubes are usually special purpose ones and then there are also those for general purpose.

Here are a few tips that may come handy when selecting a tube:

  • It is important to first make a note of the type of samples that will be used with the microcentrifuge, since different samples have different requirements.
  • The tubes themselves can be straight or curved walled. They can also be with screw tops or pop tops.
  • Their size can also vary like 0.5ml or 0.4 ml or even 0.2 ml. These are the small sized tubes and will usually require an adapter to fit in the rotor’s holes.
  • The normal sized tubes can be like 5ml and even upto 50ml.
  • Furthermore, there are several other special purpose tubes ls, like cryotubes, HPLC tubes, PCR strips, mcrotiter plates and hematocrits also called capillary tubes.
  • The rotor essentially dictates the type of tube that can be used with the mircocentrifuge. However, you do have the option of switching between rotors in some microcentrifuge devices, but not all of them.

Rotor Specifications:

  • The capacity of the rotors also vary. The capacity of the rotors can range from 24, to 30 and evey 48 standard size tubes.
  • The rotors can also be special purpose ones to deal with the tubes that contain toxic substances. Rotors with special PTFE (Teflon) coating are available for high chemical resistance.
  • The other type of rotor is the swinging bucket rotor. This one is good for density gradient centrifugation and size separation.
  • The rotors also have a speed setting and different rotors have the ability to rotate at different speeds. Each rotor will usually have a rating which dictates the speed at which they can be rotated. There are also special safety settings that prevent the rotors from rotating any faster. For example, the 48 tube rotor has a lower rating than the 24 tube rotor.

Neuation’s Centrifuge Devices:

Neuation’s Centrifuge Devices

Neuation is leading the way in the development of microcentrifuge devices. In fact they manufacture several laboratory equipment and devices. They are experts in developing new and innovative products to suite the requirements of people using such devices.

The benefit of choosing the Neuation devices for your laboratory needs is that their devices are functionally and aesthetically sound. Since they develop several laboratory equipment and also have a research division, their devices are very efficient, fast, keeping with the technological changes and above safe to use.

Things to consider while buying a Microcentrifuge

A Microcentrifuge is one of the most important equipment in a laboratory. This could be hospital laboratory a healthcare clinic or even a research laboratory. It has a vast number of applications and if considering to buy one you need to make sure you get the right type that meets your requirements.

A Microcentrifuge has a lot of features and the cost of microcentrifuge and availability will depend on the features you select. Thus to make sure you select the right set of features for your device there are a few factors mentioned in this article that you should consider.

Speed:

The speed at which the Microcentrifuge spins is of utmost importance thus this needs to be noted. Since the revolutions per minute of the device will vary in each device you need to have a set range of requirements prior to actually purchasing the device.

  • The Microcentrifuge should also have the right set of programmable features to meet your needs. The speed of the Microcentrifuge depends a lot on the type of sample that you wish to analyse. All samples have a set duration and speed that is required to be set of them.
  • It is important to note that the g-force and RCF are more important than the speed while selecting a Microcentrifuge.
  • It is possible to substitute the duration for the speed. For example if you are unable to set the desired speed on your Microcentrifuge device due to some reason or the other it is possible to lengthen the duration in order to achieve the desired result.

Temperature Sensitive Samples:

Microcentrifuges are of two different kinds; in the sense that one is refrigerated and the other is non-refrigerated. As the name suggests the refrigerated one will keep the sample placed inside very cool throughout the process thus preventing any damage to be caused to the sample.

Some of them offer additional features like last run memory or over speed running, etc. The perfect example of an industry where a refrigerated Microcentrifuge is required is the Medical Industry and especially the pathology department. If you have even been to the pathology laboratory you would have noticed that they all have cold storages used to store the blood necessary for patients. It is necessary to store blood in a cold storage in order for it to be used in blood transfusions. Again, the same thing applies while running tests on a blood sample as well. It is necessary to keep the temperature of the blood low while performing tests on it. Thus the refrigerated Microcentrifuge is perfect for this application.

Tube for Samples:

  • The tubes used in the Microcentrifuge to fill the sample are of great importance as well.
  • The tubes could be either glass or plastic, besides which the size also varies.
  • For example those devices that are used in research labs have tubes that have very small capacity.
  • The size really depends on the size allowed by your Microcentrifuge device.

If you do have a large Microcentrifuge you will usually have the option of using larger test tubes. In addition to which if you need to analyses larger samples you will need bigger test tubes for the sample.

Motor:

  • The brush-less motor is popular and is considered almost maintenance free; thus is the one you should consider opting for.
  • It is recommended that you use a brush-less motor for just about every minor requirement.
  • The brushless motor is the one to use for blood banks, chemical laboratories and just about any industry you can think off.
  • As far the bowl is concerned it would be best to opt for a stainless steel bowl ensuring easy clean out.

Display:

Some of the Microcentrifuge devices also sport a nice digital display that shows the status, time and speed. This can also be something you might want to consider.

Service:

After sales service is also important; maybe not as important as the Microcentrifuge device itself though.

Opting for a used Microcentrifuge:

Often these Microcentrifuge devices get rejected by a laboratory due to some minor defect. These devices that are thus obtained are fixed by trained technicians and refurbished; after which they function more or less as new.

  • You do have the option of opting for buying such devices if the budget is a constraint.
  • Such devices can also be considered reliable and a good alternative to a new one since usually parts warranty is offered.
  • At times you may end up with a very good quality product by paying half the price of a new one if not lesser. One of the popular places to purchase a used Microcentrifuge is ebay.com for example, which is a global website.

Specification:

The microcentrifuge specification tends to vary from device to device but here is the specifications of a Centrifuge device; which can act as a reference as to what to expect.

  • 8 Place closed rotor for 1.5/2.0 ml micro tubes (Rotor capacity)
  • 6000/2000g -> Maximum RPM/RCF(g) (Rotor)
  • Digital Display
  • Noise level of < 55db
  • 1 – 25 minutes and infinite mode (Duration settings)
  • Dimension (w x d x h) of 162 mm x 157 mm x 115 mm
  • Reduction adapters for 0.2 / 0.4 ml micro-tubes (Rotor and Adapter)

Cost of Microcentrifuge:

In order to make you aware of what features to expect in what price range a sample guide has been provided below with three different centrifuges and their price range.

  • Price range Rs 7000 to Rs. 9000: For this price range you could expect to be able to purchase a centrifuge with capacity of 4 tubes, each of 15ml with swing out head. The maximum RPM to expect would be in the range of 3100RPM, while the maximum R.C.F would be in the range of 1750g.
  • Price range Rs 20,000 to Rs 22000: For this price range it is possible to expect a centrifuge with 12 capillary tubes. The maximum speed would be up to 12000 RPM. The duration settings can range from 0 to 60 minutes.
  • Price range Rs 21,000 to Rs 23000: This is a higher price range and you can expect a higher specifications centrifuge for the price. It would be possible for you to obtain a centrifuge with about 24 capillary tubes in this price range. The speed would be approximately up to 12000 RPM and duration 0 to 60 minutes. In addition to which you can expect features like electrical lock and speed regulators.

The Safety and Maintenance of a Microcentrifuge

A microcentrifuge is one of the most common devices found in a laboratory. It is driven by a motor that spins the liquid samples at a very high speed. Thus there are many different kinds of microcentrifuges depending upon the size and capacity of the sample. It utilizes the principle of sedimentation where centripetal acceleration is used to separate substances of various different densities.

Just like any other ordinary device the microcentrifuge also demands maintenance. It is in fact necessary to carry out periodic maintenance for the microcentrifuge safety purpose. Thus it is necessary to maintain and clean the microcentrifuge so that it always operates properly. With a microcentrifuge only a few small steps are required to perform the maintenance.

Carrying out Maintenance:

  • Any extra material like plastic or broken glass (due to wear and tear of a test tube) needs to be carefully and disposed off.

  • It is also necessary to test the speeds; where the measured and rated speeds should not differ more than five percent under specific conditions. In addition to which the speeds of the microcentrifuge needs to be periodically checked using a reliable photoelectric tachometer using the guidelines of the necessary standards that are present.

  • As per the guidelines of repairing and maintaining a microcentrifuge. The microcentrifuge bowls should be cleaned with an antibacterial solution. Additionally the rotor heads and buckets need to be cleaned as well.

Sticking to the guidelines:

  • The accuracy of the microcentrifuge timer needs to be checked and verified regularly as per the given guidelines laid out.

  • The measured and programmed temperature should be within two degrees Celsius.  In case of refrigerated the microcentrifuge should be checked at least once a month in standard conditions; this is pretty much necessary to avoid future repairs of any kind.

  • As a general guideline microcentrifuges with seven hundred to fourteen hundred hours of running require minor repairs as part of the maintenance. In case of the microcentrifuge having run three thousand to four thousand hours.

  • it will require an overhaul with repairs to any damaged parts like drums, corroded shafts, rim fractures, loose shells.

  • In addition to which the bearing components need maintenance and tighten all the bolts. It would be a good idea to replace the screen, brake band, replace the rubber shock absorbers and check the bearings.

Most microcentrifuges will be accompanied along with an instruction booklet. The lubrication, maintenance and replacement of brushes should be carried out as per this instruction booklet. If this periodic maintenance like replacement of worn brushes is not carried out it there is the potential of the motor to fail which would require complete replacement.

Safety:

When it comes to using a microcentrifuge device it is of utmost importance that you consider the safety issues surrounding the operation of the device. The microcentrifuge may seem like a harmless device, but it comes with its own set of risks and issues. Thus it should only be operated by trained and official staff at all time. And even though the operator is well trained he should make it a point to read the manual prior to operating the device. There are few main points mentioned below for you to get a better idea of the risks involved while dealing with a micro-centrifuge.

Balanced Load:  It is of utmost importance that you balance the load while analysing a sample in the microcentrifuge device. The balancing of the load is achieved by placing a tube of equivalent volume in the opposite grove to your sample. The balancing should be as close as possible  if not perfect since any small difference can result in a large force imbalance at high speeds resulting in damage to the equipment and personal injury as well.

Locking Mechanism: The microcentrifuges usually have a locking mechanism that shuts down the microcentrifuge in case it is opened. Thus prior to using the device a complete check of the rotor and the lid locking mechanism should be carried out.

Hazardous Substances:  It often happens that you need to run an analysis on hazardous substances as well. Everything is fine if things run smoothly, but in case of an accident the microcentrifuge loaded with hazardous substances prove to be a big hazard to the operator and possibly the entire laboratory. Thus it is usually in best interest of the operator to wear protective glasses while operating the microcentrifuge device.

Wobbly surface:  It is imperative that you check the surface on which you wish to operate the microcentrifuge as well. If he microcentrifuge starts to wobble or the surface is not steady it is best to immediately pull the plug.

Get more information about Microcentrifuges visit – www.neuation.com or email us on –info@neuation.com or call on – +91 2764-248241.

About Author:- Johanna Williams

Johana Williams

Hi friends, I am interested in writing about science, technology & Web related stuff. Follow me on Twitter, on Google +Johanna,and/or become a friend on StumbleUpon.

What is Micro Centrifuge?

Micro-Centrifuge is a laboratory equipment that is driven by a motor spinning liquid samples at extremely high speeds. The type of the Micro Centrifuge varies depending on the size and sample capacity. There a different Micro-Centrifuges available for different applications.

Types of Micro Centrifuges:

There are many different types of Micro Centrifuge devices. They are important in providing accurate results in medical studies.

  • It is used in many different industries and medical laboratories. These Micro Centrifuges use glass tubes to store the sample for the process. Although plastic tubes tend to be less expensive.
  • There are Micro Centrifuges  that are used in blood banks and those used in chemical laboratories and then more heavy duty ones used for petroleum products.

Here a few types of Micro Centrifuges listed.

1. Preparative centrifuge :  is used to separate the organelle of a substance.

Preparative centrifuge

image courtesy : commons.wikimedia.org

2. Analytical centrifuge :  is used to a great extent in sampling substances of the object.

Analytical Centrifuge

image courtesy : ebay.com

3. Angle fixed centrifuge :  is the type of centrifuge that lets you adjust the angle of the rotor to allow proper separation of the elements of the objects under scrutiny.

Angle Fixed Centrifugeimage courtesy : hofstragroup.com

4. Swing head centrifuge :  Uses its swing rotor to break down the substance properly while getting the separation process in order.

Swing Head Centrifuge

image courtesy : rbscientific.co.uk

5. Haematocrit centrifuge : Uses a configuration system established before porches to set the amount of separation of elements than will be generated.

Haematocrit Centrifugeimage courtesy : digisystemlab.com

Using a Micro-Centrifuge:

There are a few simple rules to follow in order to ensure the safety of the Micro Centrifuge device while operating it.

  • It is necessary to counter balance any tube placed in the Micro Centrifuge. This counter balancing needs to be done according the mass and not the volume. This is required since an unbalanced Micro Centrifuge can permanently damage the device.
  • The tube for counter balancing the device is required to be placed in the opposite grove to the current one with the sample.
  • Make the appropriate setting on the Micro Centrifuge device and start it.
  • Make sure to let it complete the process before removing it; furthermore it is advisable not to touch or open the device while it is in operation.

How Micro-Centrifuge Work?

Benefits:

It can be useful in a blood bank, laboratories and even in the dairy industry.

Centrifugal Force:

  • They essentially use centrifugal force for the operation. Centrifugal force is an outward pushing force felt by bodies moving in a circular motion.
  • The unique part is that there is no actual force acting on the device and thus centrifugal force is sometimes known as a fictitious force.
  • This outward force is caused due to the combined effect of inertia and inward pushing external forces. It is only when the objects rotates does the centrifugal force come into play.
  • At times centripetal force is confused with Centrifugal force. Though centrifugal force seems very real to the objects that being rotated but does not actually exist. The fact that it is felt is because of non inertial coordinate system.

Example of the working of the force:

We can consider a real life example to better understand this so called non existence force. Consider a child on a merry go round. The child on the merry go round does not experience any real outward force but does require to exert force to stay on the merry go round. The bigger the objects or the faster the objects the greater this invisible force is.

About Company: Neuation offer a wide range of next generation microcentrifuges for various applications in research labs for processing low volume samples.

Get more information about Microcentrifuges visit – www.neuation.com or email us on – info@neuation.com or call on – +91 2764-248241.

About Author:- Johanna Williams

Johana Williams

Hi friends, I am interested in writing about science, technology & Web related stuff. Follow me on Twitter, on Google +Johanna,and/or become a friend on StumbleUpon.

What is a Micro centrifuge

There are many types of micro centrifuge. It plays an important role in providing accurate results in medical studies. It is a vital piece of equipment and is made from several different materials. The task involves separation of component products on a specimen. It is driven by a motor and spins liquid at a very high speed. The centrifuge depends on the size and capacity. Centrifugation is the process that used for separation of mixtures using centrifugal force. It is mainly used in the industries and medical laboratories. Glass centrifuge tubes can be used with almost all solvents, but tend to be more expensive. It is possible to clean them like other laboratory glassware and can be sterilized by autoclaving. Plastic centrifuge tubes tend to be less expensive

iFuge M08 – The Smart Personal MicroCentrifuges

iFuge M08 – The Smart Personal MicroCentrifuges

The load in a laboratory centrifuge must be carefully balanced which is achieved by a combination of sample and balance tube, all of which have the same weight. Small differences in mass of the load can result in a large force imbalance when the rotor is at high speed. Centrifuges have a feature where when imbalance is detected, the automatic rotor imbalance detection feature discontinues the run.

iFuge M12 – Table Top Genius

iFuge M12 – Table Top Genius

It is mandatory to check rotor and lid locking mechanisms is prior to starting the process. Centrifuge rotors should never be touched while moving, because a spinning rotor can cause serious injury. Modern centrifuges generally have features that prevent accidental contact with a moving rotor as the main lid is locked during the run.

iFuge M18P– Table Top Genius

iFuge M18P– Table Top Genius

Neuation provides a large range of Micro Centrifuges for several different kinds of applications in research labs. The Micro Centrifuge range from a personal one to a big refrigerated Micro Centrifuge that can take upto 44 tubes. There are used in cell separation.

About Author:- Johanna Sophia

Johana Sophia

Hi friends, I am interested in writing about science, technology & Web related stuff. Follow me on Twitter, on Google +Johanna, on StumbleUpon and/or become a friend on Facebook.

 

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Micro Centrifuge – Simple and Smart separation

Centrifuges are used to separate various components from a given solutions based on the densities. The device is used in laboratories, medicine manufacturing industries, blood labs and more for separation process. If you are in need to separate components from small amount of solutions then it is better to get Micro centrifuge. These centrifuges are designed with most modern features to make the separation process simple and smart. These equipments come in different models and configurations to meet the unique needs of various industries.

Tip: Make separation process simple and smart with micro centrifuges

pro-m08-img

iFuge M08

Get the best Mini microcentrifuge

Get the mini microcentrifuge from a reputed manufacturer to get the products with high end quality and standards. The equipment is made with high quality materials and innovative features to make the operation easy and effective. The equipment helps you with perfect extraction process without any errors or mistakes. Present models come with LCD screens and emergency stop buttons. Compare the features and specification of the equipment and get the model that best suits your needs.

Tip: Select the right model in Microcentrifuge that better suit your needs

Neuation is one of the reputed manufacturers of Micro Centrifuge. For more information about the equipment and to make the order online you can visit http://www.neuation.com/

Summary: Micro centrifuges are developed with innovative features for various industries and laboratories. You can select the right one to suit your needs.

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